The Indiana Myotis is a medium sized bat with longer ears and a short face. Its fur ranges from grayish to chestnut, with dull-lead colored fur at the base of the hairs and pinkish-cinnamon fur underneath the body. The Indiana Myotis diet includes a variety of aerial insects. During summer, the Indiana Myotis roosts beneath the exfoliating bark of dead trees, commonly known as snags. During winter, the species prefers to hibernate in cold sites, often limestone caves containing pools of water.

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The Indiana Myotis was one of the first bat species to be listed as endangered by the U.S. Fish and Wildlife Service. Despite measures to protect the species' winter hibernacula, populations continue to decline, due partly to its susceptibility to the fungal disease white-nose syndrome, which impacts winter populations, as well as threats like pesticide use and deforestation which limit suitable summer habitat.